Xpert Fast DNA Polymerase

For fast routine PCR Xpert Fast DNA polymerase is a robust enzyme, ideal for daily applications like genotyping and screening, amplifying with extreme speed (2 sec/kb), yield and consistency.

 

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Description

Main application: routine PCR with extreme speed

Xpert Fast DNA polymerase is a robust enzyme, ideal for daily applications like genotyping and screening, amplifying with extreme speed, yield and consistency.
Xpert Fast DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed, and can thus be cloned into TA cloning vectors.
The extreme speed of Xpert Fast DNA polymerase allows the use of an extension rate of 2 sec/kb, making this the ideal choice for consistent results in fast routine PCR amplifications.

Features

Performance highlights:
– routine amplifications with extreme speed (without performance loss)

Properties:
Amplicon size:  up to 5kb
Extension Rate: 2 sec/kb
Hotstart:            No
A-overhang:      Yes

Applications:
– Fast routine PCR
– TA Cloning
– Genotyping

Components:
– Kit version with dNTPs included (in the buffer)
– Mastermix available with (red) dye

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

Documents

Product Info Xpert Fast

Product Info Xpert Fast Mastermix 

Product Info Xpert Fast Mastermix with dye

MSDS ENG Xpert Fast DNA Polymerase

MSDS ENG Xpert Fast 2X Mastermix

MSDS ENG Xpert Fast 2X Mastermix with dye

Order Information

GE05.0500Xpert Fast DNA polymerase (dNTPs included)500U
GE05.2500Xpert Fast DNA polymerase (dNTPs included)2500U
GE12.0001Xpert Fast 2X Mastermix1mL
GE12.5001Xpert Fast 2X Mastermix5x 1mL
GE15.0001Xpert Fast 2X Mastermix with Dye1mL
GE15.5001Xpert Fast 2X Mastermix with Dye5x 1mL