Description
qACE™ Fast Probe qRT-PCR 4X Supermix enables robust and reliable qPCR detection of RNA and/or DNA targets, delivering superior amplification efficiency in single or multiplex assays, even from low copy target numbers in highly diluted samples. Using gene-specific primers, qACE™ Fast Probe qRT-PCR 4X Supermix allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably.
The 4X concentrated Supermix comprises all components, except primers and probe, for the amplification, detection and quantification of both RNA and DNA targets in qPCR reactions based on a wide range of probe-based technologies, including TaqMan, Molecular Beacons and Scorpion probes. It consists of the combination a genetically modified thermostable MMLV reverse transcriptase with improved synthesis efficiency, lacking any RNase H activity, an advanced RNase inhibitor to impede RNA degradation, and a highly efficient hot start DNA polymerase with a novel low inhibitory technology, which prevents the formation of unwanted primer-dimers and non-specific products, thus allowing for extremely high sensitivity and specificity. qACE™ Fast Probe qRT-PCR 4X Supermix is supplied with a separate vial of ROX reference dye, so it can be used with most real-time PCR instruments.
qACE™ Fast Probe qRT-PCR 4X Supermix can be used to quantify virtually any RNA target, whether using mRNA, viral RNA or total RNA as template, including extremely low-copy number targets, with minimal effort and optimization. By omitting the cDNA synthesis step, the Supermix can also be used for the for the quantification of virtually any DNA target, including extremely low-copy number targets. And by using exon-spanning primers (for RNA) mixed with intronic primers (for DNA), one can amplify RNA and DNA targets simultaneously in a multiplex RT-qPCR. Similarly, the supermix also allows for the simultaneous detection of DNA and RNA viruses (like Adenovirus (DNA genome) and Influenza (RNA genome) in a multiplex RT-qPCR reaction.