Description
The GRS Genomic DNA Kit – Yeast & Fungus – provides an efficient and fast method for the extraction and purification of high-quality genomic DNA from a large variety of yeast and fungus cultures.
Cells are efficiently lysed by vortexing in a lysis buffer mixed with zirconia/silica beads, without the need of first forming spheroplast by Zymolyase (Lyticase) treatment. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA is subsequently eluted with a low salt Elution Buffer (or TE). The entire procedure can be completed within 45 minutes without phenol extraction or ethanol precipitation, with typical DNA yields of >8µg (for 2×108 cells of S.cerevisiae). Eluted purified DNA is suitable and ready-to-use for PCR, real-time PCR, Southern Blotting, RFLP and sequencing.