Description
The GRS circulating cell-free DNA/RNA Purification Kit includes a specially developed removable extension tube that, when inserted in the mini spin column, allows for the expansion of the starting volume up to 4 ml. After lysis, antibodies and other proteins are eliminated by precipitation, and cell-free DNA/RNA is bound to the glass fiber matrix of the spin column. The mixture can be passed through the column either by centrifugation or by using a vacuum manifold system. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA/RNA is subsequently eluted with RNase-free water, and is suitable for a wide variety of downstream applications including qPCR and DNA sequencing. The entire procedure can be completed in approximately 45 minutes.
