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dsDNA Quantification Kit – Broad Range (for Fluorometer)

dsDNA Quantification Kit - Broad Range (for Fluorometer)

GRiSP´s dsDNA Quantification Kits provide an easy and fast method for the quantification of double-stranded DNA (dsDNA). This Broad Range (BR) kit was developed for use with Fluorometer (e.g.  Qubit® and Quantus™) and is accurate and reliable over a linear range from 4ng to 2000ng.

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Description

GRiSP´s dsDNA Quantification Kits provide an easy and fast method for the quantification of double-stranded DNA (dsDNA). This Broad Range (BR) kit was developed for use with Fluorometer (e.g.  Qubit® and Quantus™) and is accurate and reliable over a linear range from 4ng to 2000ng.

GRiSP´s dsDNA Quantification Kits are highly specific for double-stranded DNA. Presence of single-stranded DNA (ssDNA) or RNA does not interfere with the measurements. Other commonly present contaminants in DNA samples, such as proteins, ethanol, phenol, free nucleotides, detergents (SDS, Triton X-100), and salts (NaCl, MgCl2, acetate) also have little to no effect on the fluorescence signal.

Features

Fast, Accurate and Reliable
Broad Range from 4ng to 2000ng
Highly Specific for dsDNA
Compatible with Qubit® and Quantus™ Fluorometers

Documents

Product Info

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Order Information

GQ03.0500 dsDNA Quantification Kit – Broad Range (for Fluorometer) 500 assays

Protocols

  1. Using a clean plastic tube/container, prepare a Working Solution by mixing for each assay 1 µl of Broad Range Dye with 200 µl of Dilution Buffer (warmed up to room temperature). Do not use a glass container! (e.g. for 20 samples/standards, mix 20 µl Dye with 4 ml Buffer).
  2. Label the lids of the assay tubes for each sample and for the 2 standards. Do not label on the side as this may interfere with measurement.
  3. Pipet 190 µl of the working solution to the tubes for the 2 standards.
  4. Vortex the dsDNA standards for 2-3 seconds and add 10µl of each standard in the corresponding assay tube. Mix by vortexing for 2-3 seconds.
  5. Pipet 180-199µl of the working solution to the tubes for the samples (depending on the volume of the sample; sample volume can vary between 1-20µl as the final volume in each tube should be 200µl)
  6. Add 1-20 µl of sample to the corresponding assay tube and vortex for 2-3 seconds.
  7. Incubate at Rt for 2 minutes.
  8. Read standards and samples using the Fluorometer according to manufacturer´s instructions.