Description
Filter sterilized DMEM/F-12 supplemented with L-Glutamine. This formulation is with Phenol Red and Sodium Pyruvate and without HEPES. Detailed formulation can be found in the product information datasheet.
DMEM/F-12 with L-Glutamine
Filter sterilized DMEM/F-12 supplemented with L-Glutamine. This formulation is with Phenol Red and Sodium Pyruvate and without HEPES.
Features
DMEM/F-12
with L-Glutamine.
with Phenol Red
with Sodium Pyruvate
without HEPES
Sterile
Endotoxin-free
Protocols
DMEM/F-12 is a 1:1 mixture of DMEM (Dulbecco’s Modified Eagle Medium) and HAM´s Nutrient Mixture F-12. This combination provides a richer and more complex nutrient profile than each of the single media alone. DMEM/F-12 is widely used in cell culture as a basal medium. This particular formulation is supplemented with L-Glutamine. DMEM/F-12 is ideal for low-serum or serum-free conditions (e.g. for hPSCs, iPSCs), however, since it does not contain protein, lipids or growth factors, essential for cell proliferation, long-term viability and robust cell attachment it is often supplemented with 5-10% FBS (Fetal Bovine Serum). To maintain physiological pH, DMEM contains sodium bicarbonate and therefore must be kept in a controlled CO2-environment (5-10%). The pH indicator (phenol red) allows for monitoring pH changes from 6.2 (yellow) to 8.2 (red).
Order Information
| GTC44.0500 | DMEM/F-12 with L-Glutamine | 500mL |