Xpert Fast Hotstart DNA Polymerase

For fast amplification of complex targets Xpert Fast Hotstart DNA polymerase is a robust enzyme, suitable for the amplification of difficult targets with extreme speed (2 sec/kb), yield and specificity.

SKU N/A Category PCR Enzymes & Mixes

Description

Main application: fast amplification of complex targets

Xpert Fast Hotstart DNA polymerase is a robust enzyme, suitable for the amplification of difficult targets with extreme speed (2 sec/kb), yield and specificity.
The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. As a result, Xpert Fast Hotstart DNA Polymerase is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications.

Features

Performance highlights:
– Fast amplification of complex targets (including GC/AT-rich)
– Direct PCR from crude samples

Properties:
Amplicon size: up to 5kb
Extension Rate: 2 sec/kb
Hotstart: Yes
A-overhang: Yes

Applications:
– Direct PCR
– Fast amplification of complex targets (including GC-rich and AT-rich templates)
– Colony PCR
– Multiplex PCR

Components:
– Kit version with dNTPs included (in the buffer)
– Mastermix available with (red) dye, and without dye

Storage:-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

Documents

Product Info Xpert Fast Hotstart

Product Info Xpert Fast Hotstart Mastermix

Product Info Xpert Fast Hotstart Mastermix (2x) with dye

MSDS ENG GE25 Xpert Fast Hotstart

MSDS ENG GE35 Xpert Fast Hotstart 2X Mastermix

MSDS ENG GE45 Xpert Fast Hotstart Mastermix with Dye

Order Information

GE25.0250	Xpert Fast Hotstart DNA Polymerase (dNTPs included)	250U
GE25.1000	Xpert Fast Hotstart DNA Polymerase (dNTPs included)	1000U
GE35.0001	Xpert Fast Hotstart 2X Mastermix	1mL
GE35.5001	Xpert Fast Hotstart 2X Mastermix	5x 1mL
GE45.0001	Xpert Fast Hotstart 2X Mastermix with Dye	1mL
GE45.5001	Xpert Fast Hotstart 2X Mastermix with Dye	5x 1mL
GE45.0050	Xpert Fast Hotstart 2X Mastermix with Dye	50x 1mL