Description
Filter sterilized MEM (Minimal Essential Medium) supplemented with Earl´s Salts (also known as EMEM). This formulation is withL-Glutamine and Phenol Red and without Sodium Pyruvate. Detailed formulation can be found in the product information datasheet.
Features
EMEM
with L-Glutamine
with Phenol Red
without Sodium Pyruvate
Sterile
Endotoxin-free
Protocols
MEM (Minimal Essential Medium) supplemented with Earl´s Salts (also known as EMEM), is one of the most widely used synthetic cell culture media for supporting the growth of mammalian cells. EMEM does not contain protein, lipids or growth factors, essential for cell proliferation, long-term viability and robust cell attachment. Hence, EMEM requires supplementation, typically 10% FBS (Fetal Bovine Serum). To maintain physiological pH, MEM with Earl´s Salts contains sodium bicarbonate and therefore must be kept in a controlled CO2-environment (5-10%). The pH indicator (phenol red) allows for monitoring pH changes from 6.2 (yellow) to 8.2 (red).