Description
Filter sterilized RPMI 1640 supplemented with L-Glutamine. This formulation is with Phenol Red and without HEPES. Detailed formulation can be found in the product information datasheet.
RPMI 1640 Medium with L-Glutamine
Filter sterilized RPMI 1640 supplemented with L-Glutamine. This formulation is with Phenol Red and without HEPES.
Features
RPMI 1640
with L-Glutamine
with Phenol Red
without HEPES
Sterile
Endotoxin-free
Protocols
RPMI 1640 is widely used in cell culture suitable for supporting growth of many types of cells, including Hela, Jurkat, PC12, PBMC, MCF-7, astrocytes and carcinomas. This particular formulation is with L-glutamine and phenol Red and without HEPES.
RPMI 1640 does not contain protein, lipids or growth factors, essential for cell proliferation, long-term viability and robust cell attachment. And albeit that some cell lines may grow serum-free under specific conditions, RPMI 1640 therefore requires supplementation, typically 10% FBS (Fetal Bovine Serum). To maintain physiological pH, RPMI contains sodium bicarbonate and therefore must be kept in a controlled CO2-environment (5-10%). The pH indicator (phenol red) allows for monitoring pH changes from 6.2 (yellow) to 8.2 (red).
Order Information
| GTC42.0500 | RPMI 1640 Medium with L-Glutamine | 500mL |